mouse polyclonal anti nlrp3 (Proteintech)
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Proteintech
mouse polyclonal anti nlrp3
Mouse Polyclonal Anti Nlrp3, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+nlrp3/beta+Amyloid+1-42+Antibody/pm41643305-91-27-31
Average 95 stars, based on 154 article reviews
Mouse Polyclonal Anti Nlrp3, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+nlrp3/beta+Amyloid+1-42+Antibody/pm41643305-91-27-31
Average 95 stars, based on 154 article reviews
mouse polyclonal anti nlrp3 - by Bioz Stars,
2026-09
95/100 stars
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Transferring:Article Title: 1, 2, 4-trimethoxybenzene alleviates pain and related psychiatric disorders by inhibiting NLRP3 inflammasome activation. Article Snippet: Pain and pain-related psychiatric diseases affect approximately one-third of the global population, and effective treatment remains a lack of options.. NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is regarded as a potential therapeutic target for managing pain and related psychiatric diseases.. Our previous research reported that 1,2,4-trimethoxybenzene (1,2,4-TTB) effectively inhibited NLRP3 inflammasome activity. Membrane:Article Title: 1, 2, 4-trimethoxybenzene alleviates pain and related psychiatric disorders by inhibiting NLRP3 inflammasome activation. Article Snippet: Pain and pain-related psychiatric diseases affect approximately one-third of the global population, and effective treatment remains a lack of options.. NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is regarded as a potential therapeutic target for managing pain and related psychiatric diseases.. Our previous research reported that 1,2,4-trimethoxybenzene (1,2,4-TTB) effectively inhibited NLRP3 inflammasome activity. Blocking Assay:Article Title: 1, 2, 4-trimethoxybenzene alleviates pain and related psychiatric disorders by inhibiting NLRP3 inflammasome activation. Article Snippet: Pain and pain-related psychiatric diseases affect approximately one-third of the global population, and effective treatment remains a lack of options.. NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is regarded as a potential therapeutic target for managing pain and related psychiatric diseases.. Our previous research reported that 1,2,4-trimethoxybenzene (1,2,4-TTB) effectively inhibited NLRP3 inflammasome activity. Article Title: Cascade-Type Microglial Pyroptosis Inhibitors for Enhanced Treatment of Cerebral Ischemia-Reperfusion Injury. Article Snippet: Neuroinflammation is a critical factor in the progression of cerebral ischemia-reperfusion injury (CIRI).. Pyroptosis, which is an inflammatory form of programmed cell death, greatly amplifies neuroinflammatory processes.. It does so by promoting the release of various inflammatory contents that intensify the overall inflammatory response within the central nervous system. Article Title: Curcumin prevents neurodegeneration by blocking HDAC6-NLRP3 pathway-dependent neuroinflammation in Parkinson's disease. Article Snippet: Curcumin is a hydrophobic polyphenolic compound with potent anti-inflammatory properties.. However, whether it can achieve therapeutic effects by alleviating neuroinflammation in patients with Parkinson’s disease (PD) and its potential mechanism are still unknown.. This study explored the effects of curcumin on neuroinflammation in dopaminergic neurons and deciphered its direct target in the histone deacetylase 6 (HDAC6)– Nucleotide-binding domain, leucine-rich repeat, and pyrin domain-containing protein 3 (NLRP3) pathway, revealing the potential role of curcumin in the treatment of Parkinson’s disease. Incubation:Article Title: 1, 2, 4-trimethoxybenzene alleviates pain and related psychiatric disorders by inhibiting NLRP3 inflammasome activation. Article Snippet: Pain and pain-related psychiatric diseases affect approximately one-third of the global population, and effective treatment remains a lack of options.. NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is regarded as a potential therapeutic target for managing pain and related psychiatric diseases.. Our previous research reported that 1,2,4-trimethoxybenzene (1,2,4-TTB) effectively inhibited NLRP3 inflammasome activity. Article Title: Acetylcholine in the gingival epithelium drives the pathogenesis of periodontitis Article Snippet: .. Primary antibodies were incubated overnight at 4 °C: Article Title: Interfacial Coordination-Engineered Emulgel Modulates the Osteo-Immune Microenvironment in Periodontitis Treatment Via HIF1 Signaling Pathway-Induced Metabolic Reprogramming. Article Snippet: .. Antibodies used in western blot as follows: mouse anti-MYD88 antibody (1:2000, 67969-1-Ig, Proteintech, China), mouse anti-mTOR antibody (1:25 000, 66888-1-Ig, Proteintech, China), rabbit anti-AMPK antibody, rabbit anti-HIF-1α(1:1000, ab207442, Abcam, USA), mouse anti-NF-kB (1:1000, #Q04206, Cell Signaling Technology, USA), mouse anti-TNF-α (Proteintech, 60291-1- Ig, 1:1000), rabbit anti-iNOS (1:200, 22226-1-AP, Proteintech, China), Article Title: Cascade-Type Microglial Pyroptosis Inhibitors for Enhanced Treatment of Cerebral Ischemia-Reperfusion Injury. Article Snippet: Neuroinflammation is a critical factor in the progression of cerebral ischemia-reperfusion injury (CIRI).. Pyroptosis, which is an inflammatory form of programmed cell death, greatly amplifies neuroinflammatory processes.. It does so by promoting the release of various inflammatory contents that intensify the overall inflammatory response within the central nervous system. Article Title: Curcumin prevents neurodegeneration by blocking HDAC6-NLRP3 pathway-dependent neuroinflammation in Parkinson's disease. Article Snippet: Curcumin is a hydrophobic polyphenolic compound with potent anti-inflammatory properties.. However, whether it can achieve therapeutic effects by alleviating neuroinflammation in patients with Parkinson’s disease (PD) and its potential mechanism are still unknown.. This study explored the effects of curcumin on neuroinflammation in dopaminergic neurons and deciphered its direct target in the histone deacetylase 6 (HDAC6)– Nucleotide-binding domain, leucine-rich repeat, and pyrin domain-containing protein 3 (NLRP3) pathway, revealing the potential role of curcumin in the treatment of Parkinson’s disease. Article Title: PD-1 Attenuates Neuropathic Pain by Ameliorating NLRP3 Inflammasome-Mediated Microglia Pyroptosis. Article Snippet: Neuropathic pain lacks effective clinical treatments due to its complex pathogenesis.. Growing evidence indicates that programmed cell death receptor-1 (PD-1) plays a vital role in reducing neuropathic pain.. Recent studies have also implicated the nucleotide-binding oligomerisation domain-like receptor pyrin domain-containing 3 (NLRP3 inflammasome and its associated pyroptosis in the pathophysiology of various pain types. Western Blot:Article Title: Interfacial Coordination-Engineered Emulgel Modulates the Osteo-Immune Microenvironment in Periodontitis Treatment Via HIF1 Signaling Pathway-Induced Metabolic Reprogramming. Article Snippet: .. Antibodies used in western blot as follows: mouse anti-MYD88 antibody (1:2000, 67969-1-Ig, Proteintech, China), mouse anti-mTOR antibody (1:25 000, 66888-1-Ig, Proteintech, China), rabbit anti-AMPK antibody, rabbit anti-HIF-1α(1:1000, ab207442, Abcam, USA), mouse anti-NF-kB (1:1000, #Q04206, Cell Signaling Technology, USA), mouse anti-TNF-α (Proteintech, 60291-1- Ig, 1:1000), rabbit anti-iNOS (1:200, 22226-1-AP, Proteintech, China), Cell Culture:Article Title: Curcumin prevents neurodegeneration by blocking HDAC6-NLRP3 pathway-dependent neuroinflammation in Parkinson's disease. Article Snippet: Curcumin is a hydrophobic polyphenolic compound with potent anti-inflammatory properties.. However, whether it can achieve therapeutic effects by alleviating neuroinflammation in patients with Parkinson’s disease (PD) and its potential mechanism are still unknown.. This study explored the effects of curcumin on neuroinflammation in dopaminergic neurons and deciphered its direct target in the histone deacetylase 6 (HDAC6)– Nucleotide-binding domain, leucine-rich repeat, and pyrin domain-containing protein 3 (NLRP3) pathway, revealing the potential role of curcumin in the treatment of Parkinson’s disease. |
![( A ) to ( M ) BMDMs were cultured for 20-24 hours in BCAA-free RPMI 1640 medium supplemented with or without leucine, isoleucine, and valine at standard concentrations before priming with LPS (100 ng/mL) or vehicle control for 4 hours. Lysates were collected for RT-qPCR analysis of ( A ) Il1b and ( B ) <t>Nlrp3</t> mRNA or ( C ) Western blot analysis of pro-IL-1β, NLRP3, ASC, and caspase-1 protein. ( D ) NLRP3 protein levels were measured by optical density (OD) analysis and normalized to vehicle-treated control (Ctrl.) BMDMs that were cultured in complete medium (CM). ( E ) to ( M ) Lysates were collected for polysome profiling. ( E ) Total RNA absorbance was quantified across sucrose gradient fractions with ( F ) the abundance of polysome-associated RNA and ( G ) ratio of polysome-to-monosome-associated RNA calculated by area under the curve (AUC) analysis. ( H ) Il1b mRNA levels were quantified in unfractionated whole-cell lysate (Input) samples and ( I ) in 60S, monosome, disome, and polysome fractions, with expression normalized to an equivalent amount of Luciferase mRNA spiked into each sample. ( J ) Polysome-associated Il1b levels were measured by AUC analysis of fractions 8 to 13 (F8-F13 in [I]). ( K ) Nlrp3 mRNA levels were quantified in unfractionated whole-cell lysate (Input) samples and ( L ) in 60S, monosome, disome, and polysome fractions, with expression normalized to Luciferase mRNA. ( M ) Polysome-associated Nlrp3 levels were measured by AUC analysis of fractions 8 to 13 (F8-F13 in [L]). Data represent (A and B) the mean values of biological duplicates pooled from 4 independent experiments +/- SD, (C and E) results from 1 experiment representative of 3 independent experiments, (D) normalized densitometry values pooled from 4 independent experiments +/- SD, (F, G, J, and M) AUC data pooled from 3 independent experiments +/- SD, (H and K) the mean values of technical triplicates pooled from 3 independent experiments +/- SD, and (I and L) RT-qPCR results showing technical triplicates +/- SD from 1 experiment representative of 3 independent experiments. (A, B, D, F to H, J, K, and M) Statistical significance was determined using two-way ANOVA. ns=not significant. *p < 0.05 **p < 0.01 ***p < 0.001 ****p < 0.0001](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_88/10__64898_slash_2026__01__16__699988/10__64898_slash_2026__01__16__699988___F3.large.jpg)
